Question 1

______ techniques must be strictly adhered to prevent the accidental contamination of stock cultures.

Accepted Answer

A)  Germination
B)  Selective
C)  Differential
D)  AsepticE) None of the above
F) A) and B)

Question 2

Most bacteria reproduce by:

Accepted Answer

A)  sexual reproduction
B)  binary fission
C)  budding
D)  homologous recombinationE) All of the above
F) None of the above

Question 3

A bacterial population grows most rapidly during ________ phase.

Accepted Answer

A)  lag
B)  log
C)  stationary
D)  deathE) A) and B)
F) C) and D)

Question 4

Most bacteria:

Accepted Answer

A)  exhibit synchronous growth under natural conditions
B)  divide when a small new cell develops from the surface of an existing cell
C)  do not immediately increase in number when placed in a culture
D)  immediately begin to divide when placed in a cultureE) All of the above
F) B) and C)

Question 5

A living,growing culture whose purpose is to maintain a pure culture of an organism indefinitely is a _____.

Accepted Answer

A)  colony
B)  stock culture
C)  sporulating culture
D)  broth cultureE) A) and D)
F) All of the above

Question 6

The toxic effects of the byproducts of oxygen metabolism are reduced by which of the following

Accepted Answer

A)  Catalase
B)  Superoxide dismutase
C)  Lactase
D)  Two of theseE) C) and D)
F) B) and D)

Question 7

In the stationary phase of bacterial growth:

Accepted Answer

A)  the number of newly generated cells is greater than the number of dying cells
B)  the number of newly generated cells is less than the number of dying cells
C)  the number of newly generated cells is the same as the number of dying cells
D)  none of theseE) A) and B)
F) C) and D)

Question 8

Which of the following is considered a trace element

Accepted Answer

A)  carbon
B)  zinc
C)  sulphur
D)  phosphorousE) A) and B)
F) B) and D)

Question 9

Which type of cell will generally shift to aerobic metabolism when oxygen is available but will carry on fermentation otherwise

Accepted Answer

A)  aerotolerant anaerobes
B)  facultative anaerobes
C)  obligate anaerobes
D)  obligate aerobesE) B) and D)
F) C) and D)

Question 10

Sabourard's agar has a low pH which encourages the growth of molds and discourages the growth of bacteria.Sabourard's agar could best be described as being:

Accepted Answer

A)  selective
B)  differential
C)  complex
D)  enrichedE) A) and C)
F) A) and D)

Question 11

Within a bacterial endospore,the spore is most closely surrounded by the _____.

Accepted Answer

A)  spore membrane
B)  cortex
C)  cell membrane
D)  spore coatE) C) and D)
F) B) and C)

Question 12

A candle jar would be used for growing:

Accepted Answer

A)  Gram negative organisms
B)  fastidious organisms
C)  thermophiles
D)  microaerophilesE) None of the above
F) A) and D)

Question 13

Which of the following is most likely to have evolved to live in the deep layers of mud,where there is a complete lack of free oxygen

Accepted Answer

A)  Obligate aerobe
B)  Obligate anaerobe
C)  Facultative anaerobe
D)  Aerotolerant anaerobeE) None of the above
F) A) and C)

Question 14

Bacteria that ferment lactose grow as red colonies on MacConkey's agar while those that do not ferment lactose are transparent.MacConkey's agar can best be described as being:

Accepted Answer

A)  selective
B)  differential
C)  complex
D)  enrichedE) B) and D)
F) A) and B)

Question 15

This phase decreases at a logarithmic rate.

Accepted Answer

A)  A
B)  B
C)  C
D)  DE) B) and C)
F) A) and D)

Question 16

Which of the following counting techniques does not differentiate between live and dead bacterial cells in a culture

Accepted Answer

A)  Serial dilution
B)  Spread plate
C)  Pour plate
D)  Direct microscopic countE) C) and D)
F) All of the above

Question 17

Which of the following is not one of the phases of bacterial growth

Accepted Answer

A)  Lag phase
B)  Stationary phase
C)  Doubling phase
D)  Log phaseE) B) and C)
F) B) and D)

Question 18

Sporulation usually occurs in response to a/an _____.

Accepted Answer

A)  decrease in the level of carbon or nitrogen
B)  increase in temperature
C)  decrease in the amount of water
D)  increase in ultraviolet radiationE) B) and C)
F) All of the above

Question 19

When a bacterial cell divides into two new cells,the new cells are called:

Accepted Answer

A)  daughter cells
B)  sister cells
C)  son cells
D)  father cellsE) A) and D)
F) A) and B)

Question 20

Which of the following statements about liquid media and agar is false

Accepted Answer

A)  Both chocolate agar and blood agar contain blood.
B)  Stock cultures are never directly used for laboratory studies.
C)  A differential media will allow some bacteria to grow and stop the growth of others.
D)  A selective media will suppress the growth of some microbes.E) None of the above
F) A) and D)

Exam 7: Growth and Culturing of Bacteria

Most bacteria:

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Which of the following counting techniques does not differentiate between live and dead bacterial cells in a culture

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This phase decreases at a logarithmic rate.

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In the stationary phase of bacterial growth:

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Sporulation usually occurs in response to a/an _____.

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Which of the following is not one of the phases of bacterial growth

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When a bacterial cell divides into two new cells,the new cells are called:

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Which of the following is considered a trace element

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Most bacteria reproduce by:

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Which of the following is most likely to have evolved to live in the deep layers of mud,where there is a complete lack of free oxygen

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A candle jar would be used for growing:

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Which type of cell will generally shift to aerobic metabolism when oxygen is available but will carry on fermentation otherwise

Correct AnswerverifiedShow Answer

______ techniques must be strictly adhered to prevent the accidental contamination of stock cultures.

Correct AnswerverifiedShow Answer

Bacteria that ferment lactose grow as red colonies on MacConkey's agar while those that do not ferment lactose are transparent.MacConkey's agar can best be described as being:

Correct AnswerverifiedShow Answer

A living,growing culture whose purpose is to maintain a pure culture of an organism indefinitely is a _____.

Correct AnswerverifiedShow Answer

A bacterial population grows most rapidly during ________ phase.

Correct AnswerverifiedShow Answer

The toxic effects of the byproducts of oxygen metabolism are reduced by which of the following

Correct AnswerverifiedShow Answer

Within a bacterial endospore,the spore is most closely surrounded by the _____.

Correct AnswerverifiedShow Answer

Which of the following statements about liquid media and agar is false

Correct AnswerverifiedShow Answer

Sabourard's agar has a low pH which encourages the growth of molds and discourages the growth of bacteria.Sabourard's agar could best be described as being:

Correct AnswerverifiedShow Answer

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